Within-mice comparison of microdialysis and fiber photometry-recorded dopamine biosensor during amphetamine response

A fundamental concept in neuroscience is the transmission information neurons via neurotransmitters, -modulators, and -peptides. the past decades, the gold standard for measuring neurochemicals awake animals has been microdialysis (MD). The emergence of genetically fluorescence-based biosensors, as well as in vivo optical techniques such as fiber photometry (FP), has introduced distinct means of measuring neurotransmission. To compare MD and FP, we performed concurrent within-animal of extracellular dopamine (DA) in the dorsal striatum (DS) and after administration of amphetamine in awake, freely behaving expressing the dopamine sensor dLight1.3b. We show that despite differences, MD- and FP-based readouts of DA correlate well mice. Down-sampling of FP data showed temporal correlation MD data, with less variance observed using FP. We also present that DA fluctuations periodically reach low levels, and naïve have rapid, predrug DA dynamics measured with FP that correlate the subsequent pharmacodynamics of amphetamine as measured with and FP.