Preparation and Characterization of p-tert-Butylcalix[4]arene Modified Sol-Gel Column for Open-Tubular Capillary Electrochromatography

Using the sol-gel method, p-tert-butyl-calix[4]arene sol-gel coating capillary (BCx4-SGCC) was prepared and characterized by infrared spectroscopy and electroosmotic flow. Their separation performance was investigated by using benzenediol positional isomers and axially chiral binaphthyl derivative 2-amino-2′-hydroxy-1,1′-binaphthyl (BHA) without symmetric carbon center as probes. The migration behaviors of these analytes on the BCx4-SGCC with the open tubular capillary electrochromatography separation mode were influenced by the supermolecular interaction between calixarene and analytes. The results from the quantum chemistry calculation using DFT-B3LYP/STO-3G∗∗ base set were consistent with the migration behaviors of benzenediol and BHA.