Key result
Atherosclerotic mice show higher brain lipid-to-protein ratios and increased cholesterol on spectroscopic imaging.
Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR(-/-) mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.
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Kochan et al. (2016) studied Advanced atherosclerosis (murine model). Confocal Raman mapping and FT-IR imaging vs. C57BL/6J control mice was evaluated on Alterations in white and grey matter biochemical composition (lipid to protein ratio and total cholesterol content). Confocal Raman mapping (~0.4 μm resolution) and FT-IR imaging (~5.5 μm resolution) revealed an increased lipid-to-protein ratio and higher cholesterol in atherosclerotic ApoE/LDLR(-/-) mouse brains.
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