Association between dietary nitrate and nitrite intake and site-specific cancer risk: evidence from observational studies

// Li Xie 1, 3, * , Miao Mo 1, 3, * , Hui-Xun Jia 1, 3 , Fei Liang 1, 3 , Jing Yuan 1, 3 , Ji Zhu 1, 2, 3 1 Clinical Statistics Center, Fudan University Shanghai Cancer Center, Shanghai, China 2 Department of Radiation Oncology, Fudan University Shanghai Cancer Center, China 3 Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China * These authors have contributed equally to this work Correspondence to: Ji Zhu, email: leo.zhu@126.com Keywords: nitrate, nitrite, cancer, risk, meta-analysis Received: January 06, 2016     Accepted: July 18, 2016     Published: July 29, 2016 ABSTRACT Epidemiological studies have reported inconsistent findings on the association between dietary nitrate and nitrite intake and cancer risk. We performed a meta-analysis of epidemiological studies to summarize available evidence on the association between dietary nitrate and nitrite intake and cancer risk from published prospective and case-control studies. PubMed database was searched to identify eligible publications through April 30 th , 2016. Study-specific relative risks (RRs) with corresponding 95% confidence interval (CI) from individual studies were pooled by using random- or fixed- model, and heterogeneity and publication bias analyses were conducted. Data from 62 observational studies, 49 studies for nitrates and 51 studies for nitrites, including a total of 60,627 cancer cases were analyzed. Comparing the highest vs. lowest levels, dietary nitrate intake was inversely associated with gastric cancer risk (RR = 0.78; 95%CI = 0.67-0.91) with moderate heterogeneity ( I 2 = 42.3%). In contrast, dietary nitrite intake was positively associated with adult glioma and thyroid cancer risk with pooled RR of 1.21 (95%CI = 1.03-1.42) and 1.52 (95%CI = 1.12-2.05), respectively. No significant associations were found between dietary nitrate/nitrite and cancers of the breast, bladder, colorectal, esophagus, renal cell, non-Hodgkin lymphoma, ovarian, and pancreas. The present meta-analysis provided modest evidence that positive associations of dietary nitrate and negative associations of dietary nitrite with certain cancers.