Key result
The developed TaqMan RT-qPCR assay successfully detected 64.3 copies/µL of the FMDV target gene with high specificity and reproducibility (CV <2%), consistent with SYBR Green I RT-qPCR.
Why the study?
Foot-and-mouth disease virus poses a serious threat to the global livestock industry, and timely detection can significantly mitigate its harmful effects.
The developed TaqMan RT-qPCR assay provides a highly sensitive, specific, and reproducible method for the rapid diagnosis of foot-and-mouth disease virus.
May aid timely FMDV detection in livestock; leaves open field validation before routine adoption.
The global livestock industry is facing a serious threat from a widespread foot-and-mouth disease virus (FMDV) epidemic. The timely detection of FMDV can significantly mitigate its harmful effects. This study aimed to establish and evaluate a TaqMan fluorescence quantitative PCR assay to assess its sensitivity, specificity, reproducibility, and stability. The standard curve equation range is 6.43 × 109–6.43 × 101 copies/µL, with an R2 value of 0.996 and a standard curve equation of y = −3.586x + 36.245. The method successfully detected 64.3 copies/µL of the target gene for FMDV and exhibited high specificity for FMDV. Repeatability tests demonstrated low coefficients of variation within and between groups (<2%), indicating good reproducibility. The clinical samples analyzed using this method showed results consistent with those of the SYBR Green I RT-qPCR assay, confirming the reliability of the method. Overall, the developed test method displayed high sensitivity, specificity, reproducibility, and stability, making it suitable for the rapid diagnosis of foot-and-mouth disease in clinical settings.
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Dong et al. (2024) studied Foot-and-mouth disease virus (FMDV). TaqMan RT-qPCR assay vs. SYBR Green I RT-qPCR assay was evaluated on Detection of FMDV target gene. The developed TaqMan RT-qPCR assay successfully detected 64.3 copies/µL of the FMDV target gene with high specificity and reproducibility (CV <2%), consistent with SYBR Green I RT-qPCR.
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