What question did this study set out to answer?

Determine the structural and mechanistic basis by which terbium(III) inhibits the cleavage reaction of the hammerhead ribozyme.

January 2, 1998ScienceOpen Access

Inhibition of the Hammerhead Ribozyme Cleavage Reaction by Site-Specific Binding of Tb(III)

View Full Paper

Ask AI

Bookmark

Authors

AFAndrew L. FeigResearch Corporation for Science Advancement WSW. G. ScottUniversity of California, Riverside OUOlke C. UhlenbeckNorthwestern University

Discussion

Member takes

Implication

Crystallographic analysis demonstrates terbium(III) inhibition of hammerhead ribozyme cleavage via magnesium competition, suggesting the site functions as a conformational switch.

Key Points

Determine the structural and mechanistic basis by which terbium(III) inhibits the cleavage reaction of the hammerhead ribozyme.
Analyzed ribozyme structure and terbium(III) binding positions using X-ray crystallography.
Engineered synthetic RNA modifications adjacent to the putative terbium(III) binding site to evaluate cleavage activity.
X-ray crystallography revealed that terbium(III) binds adjacent to an essential guanosine in the catalytic core, approximately 10 angstroms from the cleavage site, competing with a single magnesium(II) ion.
Synthetically modified RNA substrate resisted terbium(III) binding and underwent cleavage in the presence of up to 20 micromolar terbium(III).

Cite This Study

Feig et al. (1998) studied this question.

synapsesocial.com/papers/6a75ebdfd01cd5d9a1707022 https://doi.org/10.1126/science.279.5347.81

View Full Paper

Ask AI

Bookmark

Implication

Crystallographic analysis demonstrates terbium(III) inhibition of hammerhead ribozyme cleavage via magnesium competition, suggesting the site functions as a conformational switch.

Inhibition of the Hammerhead Ribozyme Cleavage Reaction by Site-Specific Binding of Tb(III)

Discussion

Member takes

Implication

Key Points

Cite This Study

Inhibition of the Hammerhead Ribozyme Cleavage Reaction by Site-Specific Binding of Tb(III)

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider