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A simple bioautographic agar spray assay has been developed using the plant pathogenic fungus Valsa ceratosperma as an indicator organism for the detection and activity guided fractionation of antifungal compounds by thin-layer chromatography. Besides silica gel G60 F254 aluminium- and glass-backed plates, chemically modified silica gel (RP-18 F254S, RP-18 WF254S, RP-8, etc.) plates and various organic solvents were used to evaluate the compatibility of the agar spray technique. Two fungicides, Iprodione (Rovral®) and Mepronil (Bastak®), were tested to determine the sensitivity of the test fungus.
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Islam et al. (2003) studied this question.
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