What question did this study set out to answer?

To examine single-cell cytokine kinetics and coexpression profiles in human CD4+ T-cell clones during normal activation versus high-dose antigen tolerance induction.

June 1, 2000ImmunologyOpen Access

Single cell analysis of cytokine expression kinetics by human CD4+ T‐cell clones during activation or tolerance induction

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Authors

PPPietro PalaUniversity of Copenhagen AVAdrienne VerhoefImperial College London JLJonathan R. LambImperial College London

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Implication

In vitro study reveals distinct cytokine kinetics and refractoriness in human CD4+ T-cell clones during tolerance induction, indicating functional heterogeneity within clonal populations.

Key Points

To examine single-cell cytokine kinetics and coexpression profiles in human CD4+ T-cell clones during normal activation versus high-dose antigen tolerance induction.
Cultured human CD4+ T-cell clones (AC1.1 and HA1.7) exposed to specific peptide antigens at activating (5 μg/ml) or tolerizing (100 μg/ml) concentrations, or to phorbol 12-myristate 13-acetate and ionomycin.
Inhibited protein export using monensin or brefeldin A and measured intracellular IFN-γ, IL-4, IL-5, and TNF-α at multiple time points up to 96 hours using flow cytometry.
PMA and ionomycin induced peak TNF-α expression at 3 hours, IFN-γ in 61% of cells at 3–4 hours, and IL-4/IL-5 in 60–64% of cells at 4 hours, whereas antigen stimulation activated fewer cells with slower kinetics.
Chemical stimulation induced substantial dual expression (40% IFN-γ/IL-4 and 44% IFN-γ/IL-5), while antigen induced only 2–3% double-positive cells, demonstrating functional heterogeneity in clonal populations.
Tolerizing antigen doses elicited intracellular cytokine profiles comparable to activating doses, but both conditions triggered a transient refractory state that suppressed cytokine re-induction upon restimulation within 24–48 hours.

Cite This Study

Pala et al. (2000) studied this question.

synapsesocial.com/papers/6a7620b8cd662f67b2fbdb75 https://doi.org/10.1046/j.1365-2567.2000.00036.x

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Implication

In vitro study reveals distinct cytokine kinetics and refractoriness in human CD4+ T-cell clones during tolerance induction, indicating functional heterogeneity within clonal populations.

Single cell analysis of cytokine expression kinetics by human CD4+ T‐cell clones during activation or tolerance induction

Discussion

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Implication

Key Points

Cite This Study

Single cell analysis of cytokine expression kinetics by human CD4+ T‐cell clones during activation or tolerance induction

Discussion

Member takes

Implication

Key Points

Cite This Study

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