The development of gene transfer systems that are applicable to a wide variety of mycobacterial hosts will provide an important tool for the generation of recombinant mycobacterial candidate vaccines. Here we describe the construction of a series of hygromycin-based expression vectors that enable the expression of foreign antigens in Mycobacterium vaccae, a candidate mycobacterial delivery host. These vectors may be useful in exploring effective delivery of heterologous molecules by a variety of different mycobacterial hosts.
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Gaora et al. (2008) studied this question.