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An automated confocal Raman microspectrometer for rapid measurement of molecular concentration profiles in the skin is described. It permits the successive collection of Raman spectra at a range of depths below the skin surface. The axial resolution of the confocal Raman microspectrometer is 5.1±0.2 µm. The setup was applied to determine water concentration profiles of the stratum corneum and to determine changes therein as a result of hydration of the skin.
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Caspers et al. (2000) studied this question.
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