A simple and rapid method for extraction and HPLC-analysis of residues of enrofloxa-cin and sarafloxacin in muscle and liver from Atlantic salmon is presented. After homogenization of the tissue the fat was separated by extraction into organic solvents and the aqueous phase was analysed on the chromatograph. The method is selective and robust. The sensitivity is 5 ng/g for enrofloxacin and 10 ng/g for sarafloxacin. The method was validated and correlated with a more sensitive, but time-consuming pretreatment procedure for monitoring enrofloxacin. The method is also applicable for monitoring residues of the drugs oxolinic acid and flumequine, respectively, in Atlantic salmon.
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Hormazábal et al. (1991) studied this question.