A microanalytical method for the determination of hydroxyproline in a microquantity of connective tissues (0.05-5mg) has been developed on the basis of Prockop's conventional methods. The new method is simple, rapid, sensitive, and linear in a wider assay range (0.04-20μg), with a high reproducibility even when used by experts or novices. The present communication mainly describes the standard assay procedure of the method (protocol), which has been brought about by essential modification of the conventional method.
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Inayama et al. (1978) studied this question.