Why the study?
P-SHG microscopy was evaluated to analyze the structural consequences of sepsis on myosin filaments within skeletal muscle, as sepsis is the main cause of critical illness polyneuromyopathy.
Answers are grounded in this paper and cite their sources.
Polarization-resolved second harmonic generation microscopy can detect sub-micrometer structural alterations in myosin conformation in a rat model of sepsis, offering a potential diagnostic tool for critical illness polyneuromyopathy.
Polarization-resolved second harmonic generation (P-SHG) microscopy is able to probe the sub-micrometer structural organization of myosin filaments within skeletal muscle. In this study, P-SHG microscopy was used to analyze the structural consequences of sepsis, which is the main cause of the critical illness polyneuromyopathy (CIPNM). Experiments conducted on two populations of rats demonstrated a significant difference of the anisotropy parameter between healthy and septic groups, indicating that P-SHG microscopy is promising for the diagnosis of CIPNM. The difference, which can be attributed to a change of myosin conformation at the sub-sarcomere scale, cannot be evidenced by classical SHG imaging.
No takes yet. Share an insight, caveat, or question.
Dubreuil et al. (2018) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: