Answers are grounded in this paper and cite their sources.
RuvBL, is a member of SF6 superfamily of helicases and is conserved among the various model systems. Recently rice homolog of RuvBL has been biochemically characterized for its ATPase and DNA helicase activities, however its involvement in stress is not been studied yet. This study reports the detailed functional characterization of RuvBL homolog of Oryza sativa , under abiotic stress through transgenic approach. An improved Agrobacterium- mediated in-planta transformation method was developed in indica rice to generate the transgenic lines and study was focused on optimization of factors to achieve maximum transformation efficiency. Overexpressing OsRuvBL1a transgenics showed enhanced tolerance under in vivo salinity stress as compared to WT plants. The physiological and biochemical analysis of the OsRuvBL1a transgenic lines showed better performance under salinity and drought stresses. Several stress responsive interacting partners of OsRuvBL1a were identified using Y2H method. Working mechanism for boosting the stress tolerance by OsRuvBL1a has been proposed in this study. This integration of OsRuvBL1a gene in rice genome using in-planta transformation method helped us to achieve the abiotic stress tolerant smart crop. This study is the first direct evidence to show the novel function of RuvBL in boosting abiotic stress tolerance in plants.
No takes yet. Share an insight, caveat, or question.
Saifi et al. (2021) studied this question.