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As of January 1, 2013, the paper entitled “Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications,” by Towbin and colleagues (1), had been cited 52,488 times (ISI Web of Knowledge v5.8), placing it among the elite group of papers that have truly transformed life science research. For more than 30 years, the nitrocellulose-based Western blotting technique introduced by this paper has been a principal method for the detection of specific proteins in complex biological samples.
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Aebersold et al. (2013) studied this question.
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