Generation of human induced pluripotent stem cell lines from a fetus with congenital long QT syndrome and her healthy parents

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  Fig. 1.. Characterization of WT hiPSC lines, and a patient-derived hiPSC line with c.1898A > G mutation in KCNH2. (A–D) Phase microscopy images and immunocytochemistry of SOX2, NANOG, and OCT3/4 (in red) in the indicated hiPSC lines. Scale bars, 100 μm. (E) Quantitative PCR (qPCR) analysis of NANOG and SOX2 expression in the indicated cells. Each filled circle represents one independent experiment. Data are presented as mean ± SD. P values were calculated using one-way ANOVA with Turkey’s multiple comparisons test. (F) Fluorescence immunocytochemistry of lineage markers in derivatives differentiated from the indicated hiPSC lines. Scale bar, 50 μm. (G) Karyotype images of iPSC lines. (H) Sanger sequencing revealed a heterozygous mutation in the KCNH2 gene. Genomic DNA was extracted from the indicated hiPSC lines. (I) qPCR analysis of Sendai viral vectors was performed in the indicated cells, with three replicates for each line. (J) Mycoplasma contamination in the indicated cells was examined using the MycoAlert Mycoplasma Detection Kit, with one assay for each. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)