What question did this study set out to answer?

To encapsulate the antimicrobial peptide nisin into calcium alginate microparticles and evaluate its retention, stability against proteolytic degradation, and antibacterial effectiveness.

March 1, 1997Letters in Applied MicrobiologyOpen Access

Incorporation of nisin in micro‐particles of calcium alginate

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Authors

JWJason WanNational Institutes of Health JGJesse B. GordonHarvard University KMKaren M. MuirheadUniversity of St Andrews

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Implication

Experimental study reveals efficient encapsulation and sustained antibacterial activity of nisin in calcium alginate microparticles, suggesting improved stability for food preservation applications.

Key Points

To encapsulate the antimicrobial peptide nisin into calcium alginate microparticles and evaluate its retention, stability against proteolytic degradation, and antibacterial effectiveness.
Incorporated nisin into a calcium alginate matrix and milled the resulting preparation into microparticles smaller than 150 µm.
Characterized particle formation and structural incorporation using scanning electron microscopy.
Tested protective retention against proteolytic enzymes and measured antibacterial efficacy against Lactobacillus curvatus in MRS broth and reconstituted skim milk.
Achieved a nisin incorporation efficiency between 87% and 93% within the calcium alginate matrix.
Protected nisin from degradation, showing a marked reduction in inactivation by proteolytic enzymes compared to unencapsulated peptide.
Maintained 100% antimicrobial activity of the encapsulated nisin against Lactobacillus curvatus in both MRS broth and reconstituted skim milk.

Cite This Study

Wan et al. (1997) studied this question.

synapsesocial.com/papers/6a77377d98f24dbf64bd7532 https://doi.org/10.1046/j.1472-765x.1997.00294.x

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Implication

Experimental study reveals efficient encapsulation and sustained antibacterial activity of nisin in calcium alginate microparticles, suggesting improved stability for food preservation applications.

Incorporation of nisin in micro‐particles of calcium alginate

Discussion

Member takes

Implication

Key Points

Cite This Study

Incorporation of nisin in micro‐particles of calcium alginate

Discussion

Member takes

Implication

Key Points

Cite This Study

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