Anti-adipogenic effect of sage (Salvia officinalis L.) hot-water extract on 3T3-L1 adipocyte and HepG2 hepatocyte

Sage was extracted to hot water (S-HW) to evaluate its anti-adipogenic effect in 3 T3-L1 adipocytes and oleic acid-induced HepG2 hepatocytes. The accumulation of fat in 3 T3-L1 adipocyte and HepG2 hepatocyte was reduced by 95 and 32 %, respectively, at 100 μg/mL compared to control. The expression of adipocyte marker genes ( pparγ , c/ebp-α , srebp-1c , ap2 , fas , scd1 , acc1 , and cd36 ) in 3 T3-L1 adipocytes decreased significantly compared to adipocytes when treated with 100 μg/mL of S-HW ( P < 0.05). Similarly, the expression of adipocytes marker genes in HepG2 hepatocyte was significantly reduced at 50 μg/mL of S-HW compared to positive control ( P < 0.05). The expression of adipocyte marker proteins also decreased when 3 T3-L1 adipocyte and HepG2 hepatocyte were treated with S-HW. Therefore, S-HW has confirmed anti-adipogenic effect through the regulation of genes involved in fat accumulation in 3 T3-L1 adipocyte and HepG2 hepatocyte. • Sage was evaluated for its anti-obesity effect using hot-water extract (S-HW). • S-HW decreased fat accumulation in 3 T3-L1 adipocyte and HepG2 hepatocyte. • S-HW reduced expression of adipocyte marker gene and protein in 3 T3-L1 and HepG2. • S-HW could be used as an anti-obesity ingredient.