Identifies a specific 16-amino acid sequence (residues 792-808) on the rat alpha cardiac myosin heavy chain essential for binding myosin light chain 1.
No direct clinical application; leaves open functional relevance of this domain in human cardiac disease.
To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of subfragment 1 and the amino terminus of subfragment 2 with a full-length rat cardiac myosin light chain 1. A 16 amino acid region of the myosin heavy chain (residues 792-808) was shown to be required for myosin light chain 1 binding in an immunoprecipitation assay.
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McNally et al. (1991) studied this question.
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