Key result
TRPV4 protein is predominantly located in the nucleus of cultured neonatal rat ventricular myocytes and translocates out of the nucleus in response to hypotonic stimulation.
TRPV4 protein is predominantly localized in the nucleus of cultured neonatal rat ventricular myocytes and translocates outward in response to hypotonic stress, suggesting a potential non-channel function.
Nuclear TRPV4 localization in rat myocytes is hypothesis-generating; leaves open non-channel functions and relevance to human cardiomyocytes.
TRPV4 protein forms a Ca2+-permeable channel that is sensitive to osmotic and mechanical stimuli and responds to warm temperatures, and expresses widely in various kinds of tissues. As for cardiac myocytes, TRPV4 has been detected only at the mRNA level and there were few reports about subcellular localization of the protein. The purpose of the present study was to investigate the expression profile of TRPV4 protein in cultured neonatal rat ventricular myocytes. Using Western blots, immunofluorescence, confocal microscopy and immuno-electron microscopy, we have shown that TRPV4 protein was predominantly located in the nucleus of cultured neonatal myocytes. Furthermore, cardiac myocytes responded to hypotonic stimulation by translocating TRPV4 protein out of the nucleus. The significance and mechanism concerning the unusual distribution and translocation of TRPV4 protein in cardiac myocytes remain to be clarified.
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Zhao et al. (2012) studied this question. Hypotonic stimulation (210 mOsm/L) vs. Isotonic solution (300 mOsm/L) was evaluated on Translocation of TRPV4 protein from the nucleus (p=<0.05). TRPV4 protein is predominantly located in the nucleus of cultured neonatal rat ventricular myocytes and translocates out of the nucleus in response to hypotonic stimulation.
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