The decolorization of methyl red directed toward rapid reduction of azo group by a mixed culture of Bacillus sp. and Pseudomonas stutzeri was investigated. The improvement of the decolorization of methyl red was obtained by a mixed culture (a mixed culture 2) with particular proportion of two bacteria (Bacillus sp. and Pseudomonas stutzeri). The mixed culture 2 was grown in a LB medium with methyl red. The improvement of the decolorization of methyl red was obtained only by the growing mixed culture and not obtained by the mixture of the resting cells.
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Itoh et al. (2002) studied this question.
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