Key result
Deep sequencing of small RNAs from subclinically infected pigs revealed that the PCV2 genome does not express viral miRNAs in vivo.
PCV2 does not appear to express viral miRNAs during in vivo subclinical infection in pigs.
No change to PCV2 management in pigs; leaves open whether viral miRNAs contribute in other infection contexts or species.
Porcine circovirus type 2 (PCV2) is a ssDNA virus causing PCV2-systemic disease (PCV2-SD), one of the most important diseases in swine. MicroRNAs (miRNAs) are a new class of small non-coding RNAs that regulate gene expression post-transcriptionally. Viral miRNAs have recently been described and the number of viral miRNAs has been increasing in the past few years. In this study, small RNA libraries were constructed from two tissues of subclinically PCV2 infected pigs to explore if PCV2 can encode viral miRNAs. The deep sequencing data revealed that PCV2 does not express miRNAs in an in vivo subclinical infection.
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Núñez-Hernández et al. (2015) studied Porcine circovirus type 2 (PCV2) infection (n=6). PCV2 experimental infection vs. PBS was evaluated on Detection of PCV2-encoded viral miRNAs. Deep sequencing of small RNAs from subclinically infected pigs revealed that the PCV2 genome does not express viral miRNAs in vivo.
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