Crosslinked HA in Topical Application: The Biological Benefits of RHA Serum on Aging Signs

Background RHA Serum is a topical formulation with proven benefits on skin hydration and biomechanical properties. This study aims to evaluate its role in remodeling the extracellular matrix by stimulating the production of endogenous hyaluronic acid and collagens, thereby supporting its clinical efficacy in reducing visible signs of aging. Methods Anti‐aging effects were assessed using ex vivo human skin explants and a 56‐day, split‐face, randomized clinical study. Abdominoplasty‐derived human skin explants were treated with RHA Serum or controls and analyzed histologically and immunohistochemically for HA and collagen types I and IV expression. In parallel, 22 women aged 40–65 years applied RHA Serum or placebo daily. Skin thickness and dermal density were measured by high‐frequency ultrasound, while skin quality parameters were quantified using Visia digital imaging. Results Ex vivo analysis revealed significantly increased expression of HA and collagens I and IV in RHA Serum‐treated samples. In vivo, RHA Serum significantly improved dermal density and epidermal thickness compared to a placebo cream. Visia analysis further confirmed visible reductions in facial wrinkles and skin roughness, aligning with biological improvements observed ex vivo. Conclusion RHA Serum supports skin structure by promoting key extracellular matrix components, including collagens and endogenous HA. These biological effects translate into measurable clinical improvements. The findings support RHA Serum as a potent, evidence‐based anti‐aging topical treatment.