A simple, sensitive and reliable radioinmunoassay method for the shltaneous determination of aldosterone and deoxycorticosterone (Doc) in human plasma has been developed. The method involves extraction of plasma with methylene chloride followed by Sephadex LH-20 column chromatography prior to assay. The method is sensitive, and precision and accuracy are satisfactory. Plasma aldosterone and DOC concentration measured under different conditions are in agreement with published data.
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Verma et al. (1977) studied this question.
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