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This research aims to enhance imaging of brain functions by developing a new microscopy technique that captures multiple parameters simultaneously.

Large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) for multiparameter and quantitative brain function imaging

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Randomized trial demonstrates advanced imaging of brain functions, highlighting neural activity and neurochemical shifts.

This research aims to enhance imaging of brain functions by developing a new microscopy technique that captures multiple parameters simultaneously.
Developed large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) with 3 × 3 mm FOV and 0.7 μm resolution.
Used adaptive optics for FOV extension and a temporal multiplexing system for simultaneous two-region imaging.
Implement a field programmable gate array module for demultiplexing fluorescence signals from various brain regions.
Successfully monitored neural activities across multiple cortical regions and observed neurovascular coupling.
Achieved long-term imaging of microglial responses to neuron injury and quantified calcium concentrations in vivo.
Demonstrated a FLIM throughput of up to 15.73 megapixels/s and improved temporal resolution.

Ye et al. (2026) studied this question.

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View Full Paper

Ask AI

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Authors

Randomized trial demonstrates advanced imaging of brain functions, highlighting neural activity and neurochemical shifts.

This research aims to enhance imaging of brain functions by developing a new microscopy technique that captures multiple parameters simultaneously.
Developed large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) with 3 × 3 mm FOV and 0.7 μm resolution.
Used adaptive optics for FOV extension and a temporal multiplexing system for simultaneous two-region imaging.
Implement a field programmable gate array module for demultiplexing fluorescence signals from various brain regions.
Successfully monitored neural activities across multiple cortical regions and observed neurovascular coupling.
Achieved long-term imaging of microglial responses to neuron injury and quantified calcium concentrations in vivo.
Demonstrated a FLIM throughput of up to 15.73 megapixels/s and improved temporal resolution.

Ye et al. (2026) studied this question.

View Full Paper

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