Synthesis of cell constituents from glycine by a Pseudomonas

identified glyoxylic acid as an intermediate in the degradation of glycine by Pseudomonas fluore8cen8. Campbell (1955) prepared cell-free extracts of a species of P8eudomona8 which oxidized glycino to ammonia, carbon dioxide and water and showed that glyoxylic and formic acids were intermediates in the oxidation. Since glycine is converted into glyoxylate, it was suggested (Dagley, 1958; Callely & Dagley, 1959) that reaction sequences found in bacteria metabolizing glycollate (Korn- berg & Gotto, 1959; Kornberg & Gotto, 1961 a) and oxalate (Quayle & Keech, 1959; Quiayle, Keech & Taylor, 1961) might also be used by bacteria when growing with glycine as sole carbon source. The present work supports the view that tartronic semialdehyde is a reaction intermediate in bacterial metabolism under these conditions.