A new combination of embedding and staining methods was developed. Wich permits the visualization of the lignin distribution in the cell walls at the high resolution level of a TEM. The use of water soluble melamine resin for embedding results in extremely ultrathin sections which can be observed in the TEM without supporting films. The embedding allows a staining, specifically of lignin, with KMn O 4 : with high reproducibility. A densitometric evaluation of the micrographs reveals a great number of details about the lignin concentration within the various cell wall layers
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Maurer et al. (1990) studied this question.
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