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Electrochemiluminescence (ECL)-functionalized metal–organic frameworks (MOFs) have attracted increasing attention in biosensing in virtue of their diverse and tunable optical properties. A famous ECL luminophore, carboxyl-rich tris(4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium(II) (Ru(dcbpy) 3 2+ ), possesses the characteristics of good water solubility and excellent ECL performance and also has the potential to be the organic ligand of metal–organic frameworks. Herein, functionalized MOF nanosheets (RuMOFNSs) containing plenty of Ru(dcbpy) 3 2+ in the frameworks were synthesized in aqueous solution by a simple one-pot method. In this protocol, Ru(dcbpy) 3 2+ acted as organic ligand to coordinate with Zn 2+ originated from Zn(NO 3 ) 2, and polyvinylpyrrolidone (PVP) was used as structure-directing agent to control the formation of sheetlike structure. For practical application, a “signal-on” ECL immunosensor was designed for cardiac troponin I (cTnI) detection by employing RuMOFNSs as ECL probe. The immunosensor exhibited high sensitivity and excellent selectivity for cTnI detection in the range from 1 fg/mL to 10 ng/mL with a detection limit as low as 0.48 fg/mL. Finally, the biosensor was successfully applied for the detection of cTnI in human serum sample with satisfactory results, demonstrating its potential application in bioanalysis and clinical diagnosis.
Yan et al. (Tue,) studied this question.