Studies on the growth of a newly isolated herpes simplex virus in Vitro

Herpes Simplex Virus in vin.o I. On passage or a 11cwhy, isolated hcrpcs simplex virus (Miyama strain) on FL monolayers two subsirains were obtained from the parent virus population.One causes syncytial formatioit or Initcted cells and the o1hcr does not.2. These two types or \, irus also showed diffciences in their on^OWLh curves 'The gian! cell-producing \, irus (+CG strain) callsed early destruction or FL cells, cells initcted with.the \, irus did not form gianL cells (-Go strain), only bccamc rounded.and remaincd attachcd to the wall or the glass culture bottle for several days.Tl, e titer of intracel!ulaivirus with the +Go stiain was maxima1 16 hours artu.infection.SubscqtienL titration or cell associatcd virus was very dimcult because destructive effects became severe as iiTlbction proceeded.How-c\.er in the -Go strain virus the liter or cells continued to increase after 16 hours 3. Thc +CG strain produced giant cells in HeLa culls and L cells as well as in F1, cells but }. cells disruptcd so carly artci inkction that they dicl ITot form as large syncytia as those seen with the other two cell strains 4. \\'hen different types or virus were present in a singlc viral sample, the Iesponsc or FL cells \\'as Influenced by the viral dose 5. Histochemical changes in infected nuclei were studied SHIRO Nil AND JUNTARO KAMAHORA