Key result
Optimized Internal Amplification Controls incorporated into real-time PCR assays did not affect the limits of detection for target viruses and effectively identified failed assays.
The constructed IACs can be reliably used in real-time PCR assays to provide a robust control for routine analysis of foods for viruses without affecting the limits of detection.
May support reliable PCR virus detection in foods; leaves open prospective validation before routine adoption.
Internal amplification controls (IACs) were constructed for incorporation into real-time nucleic acid amplification assays for bovine polyomavirus, hepatitis A virus, hepatitis E virus, human adenovirus, human norovirus genogroup I, human norovirus genogroup II, murine norovirus and porcine adenovirus. The addition of optimised amounts of IAC into the assays did not affect the limits of detection for each specific target virus. A poorly performed extraction of viral nucleic acids was simulated, and the effectiveness of IACs in identifying failed assays was demonstrated. The IACs constructed in this study can be reliably used in their specific assays to provide a robust control that can be routinely applied in the analysis of foods for viruses.
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Diez‐Valcarce et al. (2011) studied Foodborne viral contamination. Internal Amplification Controls (IAC) vs. Assays without IAC was evaluated on Limit of detection (LOD) and identification of failed assays. Optimized Internal Amplification Controls incorporated into real-time PCR assays did not affect the limits of detection for target viruses and effectively identified failed assays.
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