Interactions of Flavins With Amino Acid Residues: Assessments From Spectral and Photochemical Studies

By the aid of polymerase chain reaction, the sequence coding for lectin from common wild rice was isolated and cloned into the SmaI site of pBluescript SK(+). The sequence data showed that the cloned fragment is 781bp. Like most plant lectin genes, there is no intron in the amplified sequence. The open reading frame of the fragment codes a 23kD polypeptide which contains 227 amino acid residues and there is a signal sequence with 28 amino acid residues at its NH_(2) terminus. The amplified fragment is highly homologous to the overseas published cDNA sequence with an identity of 99.48%, having three base changes at 163, 189 and 585 but without changing amino acids.