Eight Holstein Red-and-White cows were used in two squares of a 4 x 4 Latin square design experiment and fed for 84 days (weeks 4-16 of lactation) with Ca salts of fatty acids (CaSFA) of linseed oil and animal fat. Experimental diets contained 0, 3, 6 and 9% CaSFA (on DM basis) and vitamin E at doses of 40 and 168 mg kg" 1 DM of concentrate (DM). Cows were fed grass silage and 0.280 kg of concentrate per kg of milk. Average FCM yield was 24.00.5 kg/d A with 40.00.7 and 31.20.3 g/kg" 1 fat and protein, respectively. No significant effects of CaSFA or vitamin E on feed intake, milk yield, milk fat, protein or lactose contents were received. A tendency towards decreasing protein and increasing milk fat content was observed at a 9% CaSFA in the diet. The addition of CaSFA significantly (P>0.01) elevated UFA (78.07 to 67.06 g/100 g), MUFA (19.44 to 29.18 g/100 g), and PUFA (2.49 to 3.76 g/100 g). DFA (a hypocholesterolemic acid) in milk was increased from 29.35 to 42.59 g/100 g (PO.01) but total cholesterol decreased from 10.43 to 10.02 mg/100 ml of milk (P>0.05). CaSFA from linseed oil and animal fat significantly decreased the concentration of C in , C,,and C,, acids, but slightly increased the concentration of C 10 ,, C 10 " C IO , " C,", , in the milk 10' 12 14 o J 1X:I :2 7 IX:3 n3 IX:3no (P>0.01). The concentrations of EPA and DHA were not affected by treatments (P>0.05). There were no significant effects of vitamin E on the fatty acid content in milk (P>0.05). * Polfamix for ruminants, "Polfa" Kutno S.A.: Ca 160, P 60, Na 80, Mg 30, Zn 6, Cu 0.8, Co 0.03, Mn 2,1 0.1, Se 0.02 g/kg, vit. A 1 000 000, vit. D 3 120 000 IU/kg with application of 20 kg/t of concentrate mixture ** Smartamine, Rhone-Poulenc
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Brzóska et al. (1999) studied this question.