Free radical scavenging property and diuretic effect of triglize, a polyherbal formulation in experimental models

OBJECTIVE: To determine the in vitro free radical scavenging property and in vivo diuretic effect of Triglize™ , a marketed polyherbal formulation in experimental models. MATERIALS AND METHODS: The aqueous extract of polyherbal formulation (PHF) triglize was used for the experiment. The free radical scavenging property and antioxidant effect of PHF were studied by LPS-induced free radicals in rat macrophages cells and DPPH (2, 2-Diphenyl-1-Picrylhydrazyl) methods, respectively. The diuretic effect of a PHF was studied with Lipschitz model using male Wistar rats. RESULTS: PHF significantly inhibited lipopolysaccharide -induced free radicals in rat macrophages and it showed moderate antioxidant potential in DPPH model. Polyherbal formulation at 50, 200 and 400 mg/ kg significantly increased potassium excretion in urine at 0-5 h and 5-24 h. The diuretic effect of PHF was as similar as furosemide. CONCLUSION: The PHF has significant diuretic effect and free radical scavenging properties.