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November 29, 2016Science SignalingOpen Access

Oxidative stress induced virus-independent MAVS oligomerization in systemic lupus erythematosus patients, which correlated with increased type I interferon production and mitochondrial dysfunction.

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Population

Mouse embryonic fibroblasts, primary splenocytes from WT and MAVS-KO mice, and human peripheral blood…

Comparison

Chemically generated oxidative stress and… vs Untreated cells, MAVS-KO cells, cells expressing…

Design

Preclinical

Key result

Oxidative stress induced virus-independent MAVS oligomerization in systemic lupus erythematosus patients, which correlated with increased type I interferon production and mitochondrial dysfunction.

Authors

IBIwona A. BuskiewiczTMTheresa L. MontgomeryEYElizabeth C. Yasewicz

Discussion

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Overview

Oxidative stress may drive IFN signatures via MAVS in SLE; leaves open therapeutic targeting pending validation.

Study Design

Type

Observational (n=34)

Structured PICO

P
Population
17 patients with systemic lupus erythematosus and 17 healthy controls were studied to evaluate MAVS oligomerization and mitochondrial oxidative stress.
E
Exposure
Chemically generated oxidative stress (using glucose oxidase or MitoParaquat) and treatment with the mitochondria-targeted antioxidant MitoQ.
C
Comparator
Untreated cells, MAVS-KO cells, cells expressing MAVS-C79F variant, and healthy control PBMCs/plasma.
O
Outcome
MAVS oligomerization, mitochondrial membrane potential, ATP production, and type I interferon (IFN) secretion.surrogate

Oxidative stress-induced MAVS oligomerization contributes to the type I IFN signature in SLE, highlighting a potential therapeutic role for mitochondria-targeted antioxidants.

Limitations

  • Small sample size for human subject validation

Cite This Study

Buskiewicz et al. (2016) conducted an observational in Systemic lupus erythematosus (n=34). Oxidative stress vs. Healthy controls was evaluated on Spontaneous MAVS oligomerization in peripheral blood mononuclear cells. Oxidative stress induced virus-independent MAVS oligomerization in systemic lupus erythematosus patients, which correlated with increased type I interferon production and mitochondrial dysfunction.

synapsesocial.com/papers/6a7a2e2a2eb3c7e469f3e2eahttps://doi.org/10.1126/scisignal.aaf1933
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