Consecutive Signal Amplification for DNA Detection Based on De Novo Fluorophore Synthesis and Host–Guest Chemistry

Two is more than one: A method has been developed in which a DNA template triggers a Wittig reaction that leads to transfer of a benzylidene group from a DNA-linked phosphonium salt to a DNA-linked benzaldehyde. A synthetic receptor such as α-cyclodextrin then encapsulates the newly formed stilbene and triggers further signal increases.