An integrating plasmid has been used to mutagenise the gene coding for endo-β-1,3-1,4-glucanase of Bacillus subtilis. The gene, named bgl, has been mapped by PBS-1 transduction to the sacA-pureA region of the B. subtilis chromosome and is closely linked to the hutP 1 locus. The order of markers in this region is sacA 321-thiC 5-bgl-hutP 1-purA 16.
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O’Kane et al. (1985) studied this question.
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