What question did this study set out to answer?

To identify the specific factors in porcine seminal plasma that stimulate large elevations in cellular cyclic GMP and characterize their target receptors in reproductive tissues.

February 1, 1993Journal of Biological ChemistryOpen Access

Seminal plasma factors that cause large elevations in cellular cyclic GMP are C-type natriuretic peptides.

View Full Paper

Ask AI

Bookmark

Population

In vitro cell lines and porcine tissue samples

Design

Preclinical

Authors

TCT D ChrismanHoward Hughes Medical Institute SSStephanie SchulzUniversity Hospital Ulm LPLincoln R. PotterUniversity of Minnesota System

Discussion

Member takes

Implication

May indicate CNP-GC-B signaling in porcine reproduction; leaves open a fertilization role pending validation.

Key Points

To identify the specific factors in porcine seminal plasma that stimulate large elevations in cellular cyclic GMP and characterize their target receptors in reproductive tissues.
Assayed cyclic GMP elevation across multiple cell lines (Balb/3T3, NIH/3T3, Rat-2, T84, and 293 cells transfected with GC-A or GC-B receptors) following exposure to seminal plasma and CNP.
Purified active seminal factors using reverse-phase high performance liquid chromatography (RP-HPLC), followed by amino acid sequencing and antibody binding assays.
Measured CNP concentrations in seminal plasma, seminal vesicle fluid, brain, trachea, and uterus, while mapping GC-B mRNA expression and activity in uterine tissues.
Porcine seminal plasma induced a 235-fold elevation of cyclic GMP in Balb/3T3 cells and selectively activated guanylyl cyclase-B (GC-B) rather than guanylyl cyclase-A receptors.
Purified active fractions were confirmed to be C-type natriuretic peptide forms (including proCNP-103), with concentrations in seminal plasma and seminal vesicle fluid exceeding brain levels by 2,000- and 100,000-fold, respectively.
GC-B mRNA and CNP-responsive GC-B activity were localized to the uterus and oviduct, indicating functional receptor expression on the apical membrane of uterine epithelial cells.

Structured PICO

Population

In vitro cell lines (Balb/3T3, NIH/3T3, Rat-2, human T84, 293 cells stably transfected with GC-A or GC-B receptors) and porcine tissue samples (seminal plasma, seminal vesicle fluid, brain, tracheal mucosa, uterus)

Intervention

Exposure to porcine seminal plasma and C-type natriuretic peptide (CNP)

Outcome

Elevation of cellular cyclic GMP concentrationssurrogate

Porcine seminal plasma contains exceptionally high concentrations of C-type natriuretic peptide (CNP) that markedly elevate cyclic GMP via the GC-B receptor, suggesting a signaling role during fertilization.

Cite This Study

Chrisman et al. (1993) studied this question.

synapsesocial.com/papers/6a7a95f2756812e39589aa09 https://doi.org/10.1016/s0021-9258(18)53749-2

View Full Paper

Ask AI

Bookmark

Seminal plasma factors that cause large elevations in cellular cyclic GMP are C-type natriuretic peptides.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Seminal plasma factors that cause large elevations in cellular cyclic GMP are C-type natriuretic peptides.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider