Cultured embryonic skeletal muscle cells express antigenic determinants for multiple myosin types, unlike ventricular myocardium cells which predominantly express ventricular myosin.
Embryonic cell culture data warrant no clinical extrapolation; leaves open myosin isoform roles in human cardiac development.
Fluorescent antibodies against fast skeletal, slow skeletal, and ventricular myosins were applied to muscle cultures from embryonic pectoralis and ventricular myocadium of the chicken. A number of spindle-shaped mononucleated cells, presumably myoblasts, and all myotubes present in skeletal muscle cultures were labeled by all three antimyosin antisera. In contrast, in cultures from ventricular myocardium all muscle cells were labeled by anti-ventricular myosin, whereas only part of them were stained by anti-slow skeletal myosin and rare cells reacted with anti-fast skeletal myosin. The findings indicate that myosin(s) present in cultured embryonic skeletal muscle cells contains antigenic determinants similar to those present in adult fast skeletal, slow skeletal, and ventricular myosins.
No takes yet. Share an insight, caveat, or question.
Cantini et al. (1980) studied this question.