Insulin Secretion from the Isolated Pancreas in Absence of Insulinogenesis: Effect of Glucose.

The effect of dinitrophenol (DNP) on glucose stimulation of the release of immunologically measurable insulin was studied in the isolated perfused pancreas of the rat. In a 3-hour perfusion at 5 × 10−4 m DNP, protein synthesis, as measured by incorporation of valine-C14 into total pancreatic protein, was completely inhibited. The release of insulin by glucose, however, was unimpaired. Results indicate that glucose (or a product resulting from glucose metabolism) can stimulate insulin secretion directly and that a prior stimulation of insulinogenesis from amino acids is not required.