Key result
The 36-channel 4G SQUID-IMR instrument demonstrated high consistency with channel-to-channel variations of 2.09 to 5.62% and assay accuracy of 99 to 103.7% for neurodegenerative biomarkers.
Why the study?
There is clinical demand for high-throughput assays capable of detecting biomarkers at ultralow concentrations.
The newly developed 36-channel 4G SQUID-IMR instrument demonstrates high throughput, stability, and consistency for assaying ultralow concentrations of neurodegenerative biomarkers.
Answers are grounded in this paper and cite their sources.
May enable high-throughput ultralow biomarker detection in clinics; leaves open clinical validation for cardiovascular applications.
High Resolution Image Download MS PowerPoint Slide With the demands of the high-throughput assay of biomarkers of ultralow concentrations in clinics, a 36-channel instrument utilizing immunomagnetic reduction (IMR) has been developed. The instrument involves the use of a high- T c superconducting-quantum-interference-device (SQUID) magnetometer to detect the signals due to the associations between target biomarker molecules and the antibody-functionalized magnetic nanoparticles in the reagent of IMR. In addition to illustrating the design and the measurements of the instrument, the assay characterizations for eight kinds of biomarkers related to neurodegenerative disease are investigated. Furthermore, the assay results among three independent instruments were compared. For an instrument, the channel-to-channel variations in measured concentrations of biomarkers are within a range of 2.09 to 5.62%. The assay accuracy was found to be from 99 to 103.7%. The p values in measured concentrations for any of the tested biomarkers were higher than 0.05 among the three instruments. The results demonstrate high throughput, high stability, and high consistency for the SQUID-IMR instruments.
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Chen et al. (2022) studied Neurodegenerative disease biomarkers. 4G SQUID-IMR instrument was evaluated on Channel-to-channel variation and assay accuracy (recovery rate) (p=>0.05). The 36-channel 4G SQUID-IMR instrument demonstrated high consistency with channel-to-channel variations of 2.09 to 5.62% and assay accuracy of 99 to 103.7% for neurodegenerative biomarkers.
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