Isolation of Kanamycin and Paromamine Inactivated by E. Coli Carrying R Factor

Kanamycin and paromamine were inactivated in the presence of ATP and Mg++ by an enzyme solution obtained from E. coli carrying R factor. The inactivated kanamycin was isolated and it was found that the hydroxyl group on C-3 of 6-amino-6-deoxy-D-glucose moiety was phosphorylated. In the inactivated paromamine, the hydroxyl group on C-3 of D-glucosamine moiety was phosphorylated. Besides the inactivated kanamycin above described, another inactivated kanamycin containing phosphate was isolated, though it is not certain that this is the product of enzymatic inactivation.