Motivation: Ribosome profiling provides genome-wide information about translational regulation. However, there is currently no standard tool for the qualitative analysis of Ribo-seq data. We present here RiboTools, a Galaxy toolbox for the analysis of ribosome profiling (Ribo-seq) data. It can be used to detect translational ambiguities, stop codon readthrough events and codon occupancy. It provides a large number of plots for the visualisation of these events. Availability and implementation: RiboTools is available from https://testtoolshed.g2.bx.psu.edu/view/rlegendre/ribo_tools as part of the Galaxy Project, under the GPLv3 licence. It is written in python2.7 and uses standard python libraries, such as matplotlib and numpy. Contact: olivier.namy@igmors.u-psud.fr Supplementary Information: Supplementary data are available from Bioinformatics online.
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Legendre et al. (2015) studied this question.
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