TGFβR signalling controls CD103+CD11b+ dendritic cell development in the intestine

CD103 + CD11b + dendritic cells (DCs) are unique to the intestine, but the factors governing their differentiation are unclear. Here we show that transforming growth factor receptor 1 (TGFβR1) has an indispensable, cell intrinsic role in the development of these cells. Deletion of Tgfbr1 results in markedly fewer intestinal CD103 + CD11b + DCs and a reciprocal increase in the CD103 − CD11b + dendritic cell subset. Transcriptional profiling identifies markers that define the CD103 + CD11b + DC lineage, including CD101, TREM1 and Siglec-F, and shows that the absence of CD103 + CD11b + DCs in CD11c - Cre .Tgfbr1 fl/fl mice reflects defective differentiation from CD103 − CD11b + intermediaries, rather than an isolated loss of CD103 expression. The defect in CD103 + CD11b + DCs is accompanied by reduced generation of antigen-specific, inducible FoxP3 + regulatory T cells in vitro and in vivo, and by reduced numbers of endogenous Th17 cells in the intestinal mucosa. Thus, TGFβR1-mediated signalling may explain the tissue-specific development of these unique DCs.