What question did this study set out to answer?

To determine whether differences in protein-tyrosine phosphatase activities between human omental and subcutaneous adipose depots contribute to depot-specific insulin resistance.

December 1, 2001The Journal of Clinical Endocrinology & MetabolismOpen Access

Depot-Specific Variation in Protein-Tyrosine Phosphatase Activities in Human Omental and Subcutaneous Adipose Tissue: A Potential Contribution to Differential Insulin Sensitivity

View Full Paper

Ask AI

Bookmark

To determine whether differences in protein-tyrosine phosphatase activities between human omental and subcutaneous adipose depots contribute to depot-specific insulin resistance.
Assayed paired human omental and subcutaneous adipose tissue samples using an anaerobic processing technique to prevent ambient air oxidation.
Quantified baseline and dithiothreitol-reduced PTPase and PTP1B activities alongside protein expression levels of insulin receptor beta-subunit, PTP1B, and leukocyte antigen related.
Mean endogenous PTPase activity was 2.1-fold higher in omental compared with paired subcutaneous adipose tissue (P < 0.003).
Anaerobically isolated PTP1B-specific activity was 41% higher in omental tissue (P < 0.001), while total PTPase and PTP1B activities rose 42% to 71% following in vitro dithiothreitol reduction in both depots.
Protein mass of the insulin receptor beta-subunit, PTP1B, and leukocyte antigen related did not differ significantly between omental and subcutaneous depots.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Comparative tissue analysis reveals elevated protein-tyrosine phosphatase activity in human omental fat, suggesting an enzymatic mechanism for depot-specific insulin resistance.

Cite This Study

Wu et al. (2001) studied this question.

synapsesocial.com/papers/6a7bc1b324f98b6d3fc294b4 https://doi.org/10.1210/jcem.86.12.8109

View Full Paper

Ask AI

Bookmark

Implication

Comparative tissue analysis reveals elevated protein-tyrosine phosphatase activity in human omental fat, suggesting an enzymatic mechanism for depot-specific insulin resistance.

Depot-Specific Variation in Protein-Tyrosine Phosphatase Activities in Human Omental and Subcutaneous Adipose Tissue: A Potential Contribution to Differential Insulin Sensitivity

Key Points

Discussion

Implication

Cite This Study

Depot-Specific Variation in Protein-Tyrosine Phosphatase Activities in Human Omental and Subcutaneous Adipose Tissue: A Potential Contribution to Differential Insulin Sensitivity

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider