Effect of varying dietary lysine levels on growth, feed conversion, nutrient retention, lysine retention efficiency and haematological indices of Heteropneustes fossilis fry (2.97 ± 0.11 cm; 4.78 ± 0.31 g) was studied by conducting a 12-week feeding trial. Isonitrogenous (450 g kg−1 CP) and isocaloric (17.97 kJ g−1 GE) amino acid test diets with graded concentrations of l-lysine (18, 20, 22, 24, 26, 28 g kg−1 dry diet) were fed to triplicate groups of fish to apparent satiation twice daily at 17 and 17:30 h. Maximum thermal growth coefficient (TGC, 0.82), best feed conversion ratio (FCR, 1.28) highest protein retention efficiency (PRE, 36%), energy retention efficiency (ERE, 79%) and lysine retention efficiency (LRE, 75%) were noted at 24 g kg−1 lysine of dry diet. Body protein was also found to be in line with growth data and peaked at 24 g kg−1 lysine of dry diet. Similarly, superior somatic and haematological indices were exhibited by the groups fed dietary lysine at 24 g kg−1 of the dry diet. However, exponential analysis of dietary lysine intake against TGC, lysine retention and protein retention indicated that inclusion of dietary lysine in the range of 13.24–14.14 g kg−1 dry diet, corresponding to 29.42–31.42 g kg−1 dietary protein, is essential for faster growth of this fish.
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Khan et al. (2010) studied this question.
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