Twenty-one samples of commercial annatto formulations have been analyzed for color content (as bixin or norbixin) using a developed HPLC−PDA method and the results compared with those obtained using UV−vis spectrophotometric methods. HPLC−PDA provided superior qualitative and quantitative data, particularly with respect to the determination of colored degradation compounds. Two samples of norbixin of known production history were subjected to detailed HPLC analysis to identify possible differences in their colored and degradation component profiles. The samples differed significantly in their all-trans - and di- cis -norbixin isomer contents, which was indicative of their respective production histories.
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Scotter et al. (1998) studied this question.
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