Liver nitrogen movements during short-term infusion of high levels of ammonia into the mesenteric vein of sheep

Four 40 kg wethers were used in a crossover design to quantify, by arterio-venous procedures, the mass transfer of NH3, urea and amino acids (AAs) across the portal-drained viscera and the liver during a 31 min infusion of either 0 (C0) or 1100 (C1100) micromol NH4HCO3/min into the mesenteric vein. In C1100, hepatic NH3 extraction remained stable at 1214 micromol/min (1.90 micromol/min per g wet liver weight), the capacity for hepatic NH3 removal was exceeded by 654 micromol/min and the incremental (C1100-C0) urea-N release: NH3 -N removal ratio increased progressively, from 0.52 to 0.90. The NH4HCO3 infusion reduced total branched-chain AA transfer across the portal-drained viscera and total AA-N and lysine extraction by the liver. Hepatic release of glutamate was augmented ornithine switched from net release to net removal and net splanchnic release of free essential AA (44 micromol/min (sed 9.2), ) and branched-chain AA (33 micromol/min (sed 2.0), ) were reduced to 0.58 of their basal rate. The study showed that conversion of excess NH3 to urea during a short-term hepatic NH3 overload required no additional contribution of AA-N to ureagenesis; essential AA and branched-chain AA supply to non-splanchnic tissues was, however, temporarily decreased.