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The aim is to identify alpha-adrenergic receptors using the binding of a radioactive antagonist, [3H]dihydroergocryptine.

May 21, 1976Science

Alpha-Adrenergic Receptor Identification by [ ³ H]Dihydroergocryptine Binding

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The aim is to identify alpha-adrenergic receptors using the binding of a radioactive antagonist, [3H]dihydroergocryptine.
Rabbit uterine membranes were used for binding assays.
Binding reached equilibrium within 17 minutes at 25 degrees C.
The competition of adrenergic agonists for binding was analyzed.
The binding site showed affinity to adrenergic agonists in the order: epinephrine > norepinephrine > isoproterenol.
(-) stereoisomers of epinephrine and norepinephrine were 30 times more potent than their (+) counterparts.
Alpha-adrenergic antagonists effectively competed for the binding sites, but the beta-adrenergic antagonist propranolol did not.

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Randomized trial identifies alpha-adrenergic receptors in rabbit uterine membranes, suggesting a novel method for receptor study.

Williams et al. (1976) studied this question.

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