June 1, 1997The Journal of General PhysiologyOpen Access

A Highly Temperature-sensitive Proton Current in Mouse Bone Marrow–derived Mast Cells

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Population

Mouse bone marrow-derived mast cells (BMMC), immature proliferating mast cells generated by in vitro culture

Comparison

Heating from 24 to 36 degrees C, alterations in… vs Baseline conditions

Design

Preclinical

Authors

MKMiyuki KunoOsaka City University JKJunko KawawakiTokyo Metropolitan Institute of Medical Science FNFusao NakamuraToujinkai Hospital

Discussion

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Implication

Caution against clinical extrapolation from murine mast cells; leaves open its role in temperature-dependent pH homeostasis.

Structured PICO

Population

Mouse bone marrow-derived mast cells (BMMC), immature proliferating mast cells generated by in vitro culture

Intervention

Heating from 24 to 36 degrees C, alterations in intracellular/extracellular pH, ATP removal, and application of Zn2+ or bafilomycin A1

Comparator

Baseline conditions (e.g., 24 degrees C, normal pH, presence of ATP, absence of inhibitors)

Outcome

Characterization of temperature-sensitive whole cell currents and intracellular pH homeostasissurrogate

Mouse bone marrow-derived mast cells possess a highly temperature-sensitive proton conductive pathway that contributes to intracellular pH homeostasis.

Cite This Study

Kuno et al. (1997) studied this question.

synapsesocial.com/papers/6a7c459987fbbe073d26b474 https://doi.org/10.1085/jgp.109.6.731

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A Highly Temperature-sensitive Proton Current in Mouse Bone Marrow–derived Mast Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A Highly Temperature-sensitive Proton Current in Mouse Bone Marrow–derived Mast Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider