June 20, 1995Proceedings of the National Academy of SciencesOpen Access

Elimination of potassium channel expression by antisense oligonucleotides in a pituitary cell line.

View Full Paper

Ask AI

Bookmark

Population

Clonal rat pituitary cell line GH4C1

Comparison

Antisense phosphorothioate deoxyoligonucleotides… vs Nonsense deoxyoligonucleotide or untreated…

Design

Preclinical

Follow-up

48 hours

Authors

SCSungkwon ChungKyungpook National University DSDaniel SaalKaiser Permanente LKL. K. KaczmarekYale University

Discussion

Member takes

Implication

Antisense oligos enable isoform-specific current dissection in native pituitary cells; hypothesis-generating for channel roles, with no clinical implications yet.

Structured PICO

Population

Clonal rat pituitary cell line GH4C1

Intervention

Antisense phosphorothioate deoxyoligonucleotides to Kv1.5 mRNA or Kv1.4 mRNA (1 µM for 48 hours), with or without dexamethasone (1 µM for 8 hours)

Comparator

Nonsense deoxyoligonucleotide (20-mer random sequence) or untreated control cells

Outcome

Amplitude and kinetics of voltage-dependent potassium currents (delayed rectifier and rapidly inactivating currents) measured by whole-cell patch clampsurrogate

Antisense oligonucleotides successfully and specifically eliminated distinct potassium channel currents (Kv1.5 and Kv1.4) in a native pituitary cell line, demonstrating their utility in linking specific currents to channel proteins.

Cite This Study

Chung et al. (1995) studied this question.

synapsesocial.com/papers/6a7c4707d501feb4353be657 https://doi.org/10.1073/pnas.92.13.5955

View Full Paper

Ask AI

Bookmark

Implication

Antisense oligos enable isoform-specific current dissection in native pituitary cells; hypothesis-generating for channel roles, with no clinical implications yet.

Elimination of potassium channel expression by antisense oligonucleotides in a pituitary cell line.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Elimination of potassium channel expression by antisense oligonucleotides in a pituitary cell line.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider